TNF alpha antibody

Catalog No. ABIN116868

This anti-TNF alpha antibody is a Rabbit Polyclonal antibody detecting TNF alpha in WB, IHC (p), Func, EIA and IHC (fro). Suitable for Human.

Quick Overview for TNF alpha antibody (ABIN116868)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TNF alpha Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Repeated immunizations with Recombinant Human TNF alpha produced in E.coli.

Application Details

Application Notes: This IgG fraction of anti-Human TNFa has been tested for use in Neutralizations, ELISA(1/1,000-1/5,000), Immunohistochemistry, Radioimmunoassay, Immunoprecipitation,Immunohistochemistry and Immunoblotting (1/500-1/2,000). It recognizes the 17,000 MWTNFa . Reactivity in other immunoassays is unknown. Recommended Dilutions: Immunoblot using HRP Goat-anti-Rabbit IgG [H&L] and TMB as a substrate. A workingdilution range of 1/100-1/200 is suggested for this application to detect TNFa fromsupernatants or lysates of 2 x 10^6 endotoxin-stimulated human peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serumplus 10 ng/mL E. coli LPS.

Restrictions: For Research Use only

Handling

Concentration: 5.0 mg/mL (by UV Absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 without stabilizers or preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor (TNF-Alpha)

Background: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

Gene ID: 9606

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome